Interpretation der Her-2/neu-Immunhistochemie

Staining Pattern Score
 
HER2 Overexpression Assessment
(Report to treating physician)
No staining at all, or membrane staining in less than 10% of 
the tumour cells is observed.  
0 Negative
A faint/barely perceptible membrane staining is detected in 
more than 10% of the tumour cells. The cells are only stained 
in part of their membrane.  
1+ Negative
A weak to moderate staining of the entire membrane is 
observed in more than 10% of the tumour cells.  
2+ Weakly positive
A strong staining of the entire membrane is observed in 
more than 10% of the tumour cells.  
3+ Strongly positive

 

score: 0 - negativ

score: 0 - negativ

Hercep0 1 Hercep0 2

score 1+ - negativ

score 1+ - negativ

Hercep1 1 Hercep1 2

score 2+ - schwach positiv

score 2+ - schwach positiv

score 2+ - schwach positiv

Hercep2 1 Hercep2 2 Hercep2 3

 score 3+ - stark positiv 

Score 3+ - stark positiv

score 3+ - stark positiv

Hercep3 1 Hercep3 2

Photos by James Thompson, MD, PhD, Director of Pathology, Biopharmaceutical Services, Impath Lab., Frolian Espinoza, MD, Molecular Tissue Pathology, 
Quest Diagnostics/Nichols Institute and DAKO 

 

Wann ist eine HER-2/neu-FISH-Analyse nötig ?

HercepFISH

nach: Diaz NM. Laboratory testing for HER2/neu in breast carcinoma: an evolving strategiy to predict response
to targeted therapy. Cancer Control 2001;8:415-418

Hercept-FISH-negativ  Hercept-FISH-positiv

HER2 FISH - kein amplifiziertes HER2-Gen

HER2 FISH - amplifiziertes HER2-Gen

 

CISH analog zu FISH ohne qualitativen Unterschied:

HercepCISH

Interpretation:

Anzahl der Signale pro Kern: Mittelwert aus 2x20 ausgezählten Tumorzellkernen.

Kriterium Signale pro Kern
normal < oder =4
low level-Amplifikation 5-10
high level-Amplifikation mehr als 10 oder große Cluster