Interpretation der Her-2/neu-Immunhistochemie
| Staining Pattern | Score |
HER2 Overexpression Assessment
(Report to treating physician)
|
| No staining at all, or membrane staining in less than 10% of the tumour cells is observed. |
0 | Negative |
| A faint/barely perceptible membrane staining is detected in more than 10% of the tumour cells. The cells are only stained in part of their membrane. |
1+ | Negative |
| A weak to moderate staining of the entire membrane is observed in more than 10% of the tumour cells. |
2+ | Weakly positive |
| A strong staining of the entire membrane is observed in more than 10% of the tumour cells. |
3+ | Strongly positive |
score: 0 - negativ |
score: 0 - negativ |
|
 |
 |
|
score 1+ - negativ |
score 1+ - negativ |
|
 |
 |
|
score 2+ - schwach positiv |
score 2+ - schwach positiv |
score 2+ - schwach positiv |
 |
 |
 |
score 3+ - stark positiv |
Score 3+ - stark positiv |
score 3+ - stark positiv |
 |
 |
|
Photos by James Thompson, MD, PhD, Director of Pathology, Biopharmaceutical Services, Impath Lab., Frolian Espinoza, MD, Molecular Tissue Pathology,
Quest Diagnostics/Nichols Institute and DAKO
Wann ist eine HER-2/neu-FISH-Analyse nötig ?
nach: Diaz NM. Laboratory testing for HER2/neu in breast carcinoma: an evolving strategiy to predict response
to targeted therapy. Cancer Control 2001;8:415-418
HER2 FISH - kein amplifiziertes HER2-Gen |
HER2 FISH - amplifiziertes HER2-Gen |
CISH analog zu FISH ohne qualitativen Unterschied:
Interpretation:
Anzahl der Signale pro Kern: Mittelwert aus 2x20 ausgezählten Tumorzellkernen.
| Kriterium | Signale pro Kern |
| normal | < oder =4 |
| low level-Amplifikation | 5-10 |
| high level-Amplifikation | mehr als 10 oder große Cluster |
